Erp44^Gt(XB599)Byg
Gene trapped Allele Detail

Summary

• Symbol: Erp44^Gt(XB599)Byg
• Name: endoplasmic reticulum protein 44; gene trap XB599, BayGenomics
• MGI ID: MGI:4124445
• Gene: Erp44 Location: Chr4:48193323-48279558 bp, - strand Genetic Position: Chr4, 26.09 cM
• Alliance: Erp44^Gt(XB599)Byg page

Mutation origin

• Mutant Cell Line: XB599
• Germline Transmission: Earliest citation of germline transmission: J:244948
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0pfs Vector Type: gene trap
• Mutation details: The gene trap vector inserted into genomic intron 1. An exon1-lacZ fusion mRNA product of 294 bp was detected by reverse-transcriptase polymerase chain reaction (RT-PCR). Wild type mRNA and protein levels were undetectable in homozygous mutants as assessed by RT-PCR and immunoblot analysis. (J:244948)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XB599	CC156313 ; (MGI Seq Detail)	5' RACE	Chr4:48279384-48279596 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  12 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:

133 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Erp44 Mutation: 45 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)