Chmp1a^Gt(XC472)Byg
Gene trapped Allele Detail

Summary

• Symbol: Chmp1a^Gt(XC472)Byg
• Name: charged multivesicular body protein 1A; gene trap XC472, BayGenomics
• MGI ID: MGI:4124823
• Gene: Chmp1a Location: Chr8:123931003-123939502 bp, - strand Genetic Position: Chr8, 72.08 cM
• Alliance: Chmp1a^Gt(XC472)Byg page

Mutation origin

• Mutant Cell Line: XC472
• Germline Transmission: Earliest citation of germline transmission: J:271138
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: A gene trap cassette inserted in intron 1 contained a strong splice acceptor from En2 fused to the coding sequence for beta-galactosidase. Western blot analysis confirmed the absence of protein expression in homozygous mutant mice. (J:271138)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XC472	CC178156 ; (MGI Seq Detail)	5' RACE	Chr8:123939405-123939500 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  5 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	15 assay results
In Structures Affected by this Mutation:	11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Chmp1a Mutation: 15 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)