Ppib^{Gt(RST139)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Ppib^{Gt(RST139)Byg}
• Name: peptidylprolyl isomerase B; gene trap RST139, BayGenomics
• MGI ID: MGI:4125284
• Synonyms: Ppib^GT
• Gene: Ppib Location: Chr9:65967504-65973905 bp, + strand Genetic Position: Chr9, 35.69 cM
• Alliance: Ppib^{Gt(RST139)Byg} page

Mutation origin

• Mutant Cell Line: RST139
• Germline Transmission: Earliest citation of germline transmission: J:226318
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0TMpfs Vector Type: secretory trap
• Mutation details: The gene trap construct inserted into intron 1 and consisted of a splice acceptor sequence from the mouse En2 gene, followed by a beta-galactosidase/neomycin (beta-geo) reporter-selection cassette and SV40 polyadenylation signal. The insertion of the gene trap vector inserted 123 bp from the 5-end of the 1066 bp intron 1. The insertional mutation interrupts transcription of the gene in cells and tissues isolated from homozygous mutant mice. Expression of the gene was reduced in cells and tissues heterozygous for the gene-trapped allele, and completely absent from homozygous cells and tissues. (J:226318)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RST139	CC178664 ; (MGI Seq Detail)	5' RACE	Chr9:65967500-65967679 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  20 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Disease models

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ppib Mutation: 18 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 7 reference(s)