Rnf7^Gt(XE423)Byg
Gene trapped Allele Detail

Summary

• Symbol: Rnf7^Gt(XE423)Byg
• Name: ring finger protein 7; gene trap XE423, BayGenomics
• MGI ID: MGI:4125996
• Synonyms: Sag^gt
• Gene: Rnf7 Location: Chr9:96352990-96360728 bp, - strand Genetic Position: Chr9, 50.63 cM, cytoband D2.3
• Alliance: Rnf7^Gt(XE423)Byg page

Mutation origin

• Mutant Cell Line: XE423
• Germline Transmission: Earliest citation of germline transmission: J:164457
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: The gene trap vector inserted in the second intron disrupting the transcript and producing a truncated fusion protein that lacks the RING domain. Immunoblot analysis confirmed the absence of protein expression in homozygous embryos. (J:178926)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XE423	CC183161 ; (MGI Seq Detail)	5' RACE	multiple
  * GRCm39; (strand of gene trap mutagenesis)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rnf7 Mutation: 17 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 5 reference(s)