Pum1^Gt(XE002)Byg
Gene trapped Allele Detail

Summary

• Symbol: Pum1^Gt(XE002)Byg
• Name: pumilio RNA-binding family member 1; gene trap XE002, BayGenomics
• MGI ID: MGI:4126030
• Gene: Pum1 Location: Chr4:130390632-130508875 bp, + strand Genetic Position: Chr4, 63.43 cM
• Alliance: Pum1^Gt(XE002)Byg page

Mutation origin

• Mutant Cell Line: XE002
• Germline Transmission: Earliest citation of germline transmission: J:226959
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: Trapped gene association determined by genome coordinate overlap and strand. A gene trap vector (pGT1Lxf) that contains a splice-acceptor sequence upstream of the reporter gene, beta-geo inserted inside the intron between exon 2 and exon 3. Sequence analysis showed that the gene trap inserted about 10-kb downstream of exon 2, leading to a new splicing product composed of exon 1, exon 2, and beta-galactosidase gene. RT-PCR and sequences confirmed the presence of a fusion mRNA transcript. (J:226959)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XE002	CC183197 ; (MGI Seq Detail)	5' RACE	Chr4:130390686-130396512 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  289 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pum1 Mutation: 169 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)