Del(10Mcm9-Asf1a)1Byg
Gene trapped Allele Detail

Summary

• Symbol: Del(10Mcm9-Asf1a)1Byg
• Name: gene trap XE518 induced deletion 1, BayGenomics
• MGI ID: MGI:4126239
• Synonyms: Mcm9/Asf1a^Gt(XE518)Byg, Mcm9^XE518, XE
• Gene: Del(10Mcm9-Asf1a)1Byg Location: Chr10:53472696-53496109 bp Genetic Position: Chr10, Syntenic

Del(10Mcm9-Asf1a)1Byg/Del(10Mcm9-Asf1a)1Byg embryos are developmentally arrested at E8.5

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: XE518
• Germline Transmission: Earliest citation of germline transmission: J:177420
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout)
• Mutations: Insertion of gene trap vector, Intergenic deletion, Intragenic deletion Vector: pGT1Lxf Vector Type: gene trap
• Del(10Mcm9-Asf1a)1Byg involves 2 genes/genome features (Mcm9, Asf1a) View all
• Mutation details: The gene trap vector inserted in intron 4 of Mcm9 causing a deletion downstream of exon 4 including all of Asf1a. RT-PCR confirmed the absence of mRNA for both Asf1a and Mcm9 in homozygous embryos. (J:177420)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XE518	CC183442 ; (MGI Seq Detail)	5' RACE	Chr10:53496230-53501907 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  1 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Schematic of the insertion sites of the Asf1a^{Gt(AL0673)Wtsi}, Del(10Mcm9-Asf1a)1Byg, Mcm9^{Gt(AW0655)Wtsi}, and Mcm9^Gt(XG743)Byg gene traps

Expression

In Mice Carrying this Mutation:	6 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Del(10Mcm9-Asf1a)1Byg

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Del(10Mcm9-Asf1a)1Byg Mutation: 0 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)