Piezo1^{Gt(RST343)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Piezo1^{Gt(RST343)Byg}
• Name: piezo-type mechanosensitive ion channel component 1; gene trap RST343, BayGenomics
• MGI ID: MGI:4126380
• Synonyms: Piezo1^gt
• Gene: Piezo1 Location: Chr8:123208437-123278068 bp, - strand Genetic Position: Chr8, 71.31 cM
• Alliance: Piezo1^{Gt(RST343)Byg} page

Growth retardation and pericardial effusion in Piezo1^{Gt(RST343)Byg}/Piezo1^{Gt(RST343)Byg} embryos

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: RST343
• Germline Transmission: Earliest citation of germline transmission: J:212179
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0TMpfs Vector Type: secretory trap
• Mutation details: A gene trap vector randomly integrated in the intron between exons 18 and 19 of the gene. The gene trap cassette contains a splice acceptor that co-opts the endogenous splicing of the mRNA transcript, leading to an in-frame fusion protein containing the first 823 residues of the encoded protein and beta-galactosidase. This gene trap vector also provides a human placental alkaline phosphatase (PLAP) reporter gene expressed through an internal ribosome entry site (IRES). Quantitative PCR analysis using a probe downstream of the gene trap integration site confirmed loss of wild-type transcript in mutant embryos. (J:212179)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RST343	CC183592 ; (MGI Seq Detail)	5' RACE	Chr8:123223478-123224229 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  22 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Generation of the Piezo1^{Gt(RST343)Byg} allele

Expression

In Mice Carrying this Mutation:	28 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Piezo1 Mutation: 89 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 7 reference(s)