Piezo1Gt(RST343)Byg
Gene trapped Allele Detail
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Symbol: |
Piezo1Gt(RST343)Byg |
Name: |
piezo-type mechanosensitive ion channel component 1; gene trap RST343, BayGenomics |
MGI ID: |
MGI:4126380 |
Synonyms: |
Piezo1gt |
Gene: |
Piezo1 Location: Chr8:123208437-123278068 bp, - strand Genetic Position: Chr8, 71.31 cM
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Alliance: |
Piezo1Gt(RST343)Byg page
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Growth retardation and pericardial effusion in Piezo1Gt(RST343)Byg/Piezo1Gt(RST343)Byg embryos
Show the 3 phenotype image(s) involving this allele.
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Mutant Cell Line: |
RST343 |
Germline Transmission: |
Earliest citation of germline transmission:
J:212179
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Parent Cell Line: |
Other (see notes) (ES Cell)
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Strain of Origin: |
129P2/OlaHsd
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Allele Type: |
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Gene trapped (Reporter) |
Mutation: |
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Insertion of gene trap vector
Vector: pGT0TMpfs
Vector Type: secretory trap
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Mutation details: A gene trap vector randomly integrated in the intron between exons 18 and 19 of the gene. The gene trap cassette contains a splice acceptor that co-opts the endogenous splicing of the mRNA transcript, leading to an in-frame fusion protein containing the first 823 residues of the encoded protein and beta-galactosidase. This gene trap vector also provides a human placental alkaline phosphatase (PLAP) reporter gene expressed through an internal ribosome entry site (IRES). Quantitative PCR analysis using a probe downstream of the gene trap integration site confirmed loss of wild-type transcript in mutant embryos.
(J:212179)
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Sequence Tags: |
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Sequence tag details (1 tag)
Tag ID |
GenBank ID |
Method |
Tag Location (trapped strand)* |
Select |
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Genome Context: |
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Genome Browser view of this mutation
22 gene trap insertions have trapped this gene
View all gene trap sequence tags in this region
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Generation of the Piezo1Gt(RST343)Byg allele |
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View phenotypes and curated references for all genotypes (concatenated display).
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All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.
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Original: |
J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008; |
All: |
7 reference(s) |
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