About   Help   FAQ
Piezo1Gt(RST343)Byg
Gene trapped Allele Detail
Summary
Symbol: Piezo1Gt(RST343)Byg
Name: piezo-type mechanosensitive ion channel component 1; gene trap RST343, BayGenomics
MGI ID: MGI:4126380
Synonyms: Piezo1gt
Gene: Piezo1  Location: Chr8:123208437-123278068 bp, - strand  Genetic Position: Chr8, 71.31 cM
Alliance: Piezo1Gt(RST343)Byg page
Growth retardation and pericardial effusion in Piezo1Gt(RST343)Byg/Piezo1Gt(RST343)Byg embryos

Show the 3 phenotype image(s) involving this allele.

Mutation
origin
Mutant Cell Line:  RST343
Germline Transmission:  Earliest citation of germline transmission: J:212179
Parent Cell Line:  Other (see notes) (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Gene trapped (Reporter)
Mutation:    Insertion of gene trap vector     Vector: pGT0TMpfs     Vector Type: secretory trap
 
Mutation detailsA gene trap vector randomly integrated in the intron between exons 18 and 19 of the gene. The gene trap cassette contains a splice acceptor that co-opts the endogenous splicing of the mRNA transcript, leading to an in-frame fusion protein containing the first 823 residues of the encoded protein and beta-galactosidase. This gene trap vector also provides a human placental alkaline phosphatase (PLAP) reporter gene expressed through an internal ribosome entry site (IRES). Quantitative PCR analysis using a probe downstream of the gene trap integration site confirmed loss of wild-type transcript in mutant embryos. (J:212179)
Sequence Tags: 
Sequence tag details (1 tag)
Genome Context: 
Genome Browser view of this mutation
Generation of the Piezo1Gt(RST343)Byg allele
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 9 assay results
1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation: 2 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Piezo1 Mutation:  89 strains or lines available
Notes
All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.
References
Original:  J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008;
All:  7 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
10/29/2024
MGI 6.24
The Jackson Laboratory