Mcm9^Gt(XG743)Byg
Gene trapped Allele Detail

Summary

• Symbol: Mcm9^Gt(XG743)Byg
• Name: minichromosome maintenance 9 homologous recombination repair factor; gene trap XG743, BayGenomics
• MGI ID: MGI:4126968
• Synonyms: Mcm9^XG743, XG
• Gene: Mcm9 Location: Chr10:53412411-53506535 bp, - strand Genetic Position: Chr10, 27.21 cM, cytoband B3
• Alliance: Mcm9^Gt(XG743)Byg page

Germ-cell depletion and loss of spermatogonial stem cells in Mcm9^Gt(XG743)Byg/Mcm9^Gt(XG743)Byg males

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: XG743
• Germline Transmission: Earliest citation of germline transmission: J:177420
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: The gene trap vector inserted downstream of exon 7. RT-PCR analysis detected a slight amount of the long isoform (less than 3% of wild-type levels) and reduced levels of the short isoform. Expression of Asf1a is about 75% of wild-type levels. (J:177420)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XG743	CC200116 ; (MGI Seq Detail)	5' RACE	Chr10:53487449-53487568 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  26 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Schematic of the insertion sites of the Asf1a^{Gt(AL0673)Wtsi}, Del(10Mcm9-Asf1a)1Byg, Mcm9^{Gt(AW0655)Wtsi}, and Mcm9^Gt(XG743)Byg gene traps

Disease models

Expression

In Mice Carrying this Mutation:	20 assay results
In Structures Affected by this Mutation:	7 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Mcm9^Gt(XG743)Byg

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mcm9 Mutation: 75 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 4 reference(s)