Pofut2^{Gt(RST434)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Pofut2^{Gt(RST434)Byg}
• Name: protein O-fucosyltransferase 2; gene trap RST434, BayGenomics
• MGI ID: MGI:4127173
• Synonyms: RST434
• Gene: Pofut2 Location: Chr10:77095052-77105409 bp, + strand Genetic Position: Chr10, 39.72 cM
• Alliance: Pofut2^{Gt(RST434)Byg} page

Mutation origin

• Mutant Cell Line: RST434
• Germline Transmission: Earliest citation of germline transmission: J:165751
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0TMpfs Vector Type: secretory trap
• Mutation details: A gene trap vector insertion occurred within exon 6 and resulted in an inframe fusion between Pofut2 (exons 1 through 5) and beta-gal-neomycin. The resulting fusion protein lack TSR-specific O-fucosyltransferase activity. It is a null allele. (J:165751)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RST434	CC200338 ; (MGI Seq Detail)	5' RACE	Chr10:77096593-77100855 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  8 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	72 assay results
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Pofut2 Mutation: 29 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 4 reference(s)