Sec23a^{Gt(RRE297)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Sec23a^{Gt(RRE297)Byg}
• Name: SEC23 homolog A, COPII coat complex component; gene trap RRE297, BayGenomics
• MGI ID: MGI:4128052
• Synonyms: Sec23a^gt
• Gene: Sec23a Location: Chr12:59005170-59058803 bp, - strand Genetic Position: Chr12, 25.97 cM
• Alliance: Sec23a^{Gt(RRE297)Byg} page

Mutation origin

• Mutant Cell Line: RRE297
• Germline Transmission: Earliest citation of germline transmission: J:237614
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT2Lxf Vector Type: gene trap
• Mutation details: A gene trap vector inserted into intron 2. The resulting fusion transcript of the first two exons of the gene (encoding the first 73 amino acids) with the beta-geo cassette produces a chimeric protein missing over 90% of the coding region, which is expected to be nonfunctional. Western blot analysis using an antibody recognizing an internal epitope near the C-terminus confirmed the absence of protein expression in homozygous mutant embryonic fibroblast cell lysates. (J:237614)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRE297	CC249458 ; (MGI Seq Detail)	5' RACE	Chr12:59053776-59058770 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  16 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sec23a Mutation: 47 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)