Chmp2b^Gt(XL952)Byg
Gene trapped Allele Detail

Summary

• Symbol: Chmp2b^Gt(XL952)Byg
• Name: charged multivesicular body protein 2B; gene trap XL952, BayGenomics
• MGI ID: MGI:4128363
• Gene: Chmp2b Location: Chr16:65336014-65359612 bp, - strand Genetic Position: Chr16, 37.26 cM, cytoband C1.3
• Alliance: Chmp2b^Gt(XL952)Byg page

Mutation origin

• Mutant Cell Line: XL952
• Germline Transmission: Earliest citation of germline transmission: J:219860
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Hypomorph)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: A gene trap vector (pGT0Lxf) composed of a splice acceptor site (SA), a beta-galactosidase-neomycin fusion protein (b-geo) and a polyA tail (pA) was inserted into intron 2. Quantification of protein levels showed that the knockout is incomplete leading to an 84% reduction in protein levels. (J:221848)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XL952	CC325151 ; (MGI Seq Detail)	5' RACE	Chr16:65347849-65359406 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  2 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	4 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Chmp2b Mutation: 24 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 6 reference(s)