Zfp105^{Gt(TEA059)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Zfp105^{Gt(TEA059)Byg}
• Name: zinc finger protein 105; gene trap TEA059, BayGenomics
• MGI ID: MGI:4128645
• Synonyms: Zfp105:LacZ, Zfp105^LacZ
• Gene: Zfp105 Location: Chr9:122752137-122760093 bp, + strand Genetic Position: Chr9, 73.23 cM
• Alliance: Zfp105^{Gt(TEA059)Byg} page

Presence of undifferentiated spermatogenic cells in the lumen of Zfp105^{Gt(TEA059)Byg}/Zfp105^{Gt(TEA059)Byg} testes and epididymides

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: TEA059
• Germline Transmission: Earliest citation of germline transmission: J:160251
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: The gene-trap vector contains a splice-acceptor site upstream of a LacZ/Neomycin (Neo) (a fusion of LacZ and Neo). The insertion of the vector into intron 3 produces an exon 1-3-LacZ/Neo fusion protein. Northern blot analysis using radiolabeled probes against exon 4 showed the complete loss of RNA transcripts in homozygous testes. LacZ staining showed that robust expression in the testis with dominant expression in the spermatocytes (especially pachytene spermatocytes). Weak LacZ staining was also observed in a few follicles of ovaries. (J:160251)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  TEA059	CC325489 ; (MGI Seq Detail)	5' RACE	Chr9:122752245-122755515 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  7 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Generation of the Zfp105^{Gt(TEA059)Byg} allele

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zfp105 Mutation: 25 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)