Egln1^{Gt(RRG405)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Egln1^{Gt(RRG405)Byg}
• Name: egl-9 family hypoxia-inducible factor 1; gene trap RRG405, BayGenomics
• MGI ID: MGI:4128927
• Synonyms: Hif-p4h-2^gt
• Gene: Egln1 Location: Chr8:125635326-125676063 bp, - strand Genetic Position: Chr8, 72.86 cM
• Alliance: Egln1^{Gt(RRG405)Byg} page

Mutation origin

• Mutant Cell Line: RRG405
• Germline Transmission: Earliest citation of germline transmission: J:162597
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Hypomorph)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The beta-geo containing gene trap vector inserted between exon 1 and 2. Reduced protein expression was confirmed by western blot analysis on heart, skeletal muscle, kidney, spleen, lung, bladder, brain, and liver extracts. This allele is described as hypomorphic. (J:162597)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRG405	CC325801 ; (MGI Seq Detail)	5' RACE	Chr8:125674971-125675322 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  2 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Generation of the Egln1^{Gt(RRG405)Byg} allele

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Egln1 Mutation: 22 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 14 reference(s)