Ppp2r5d^{Gt(RRG285)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Ppp2r5d^{Gt(RRG285)Byg}
• Name: protein phosphatase 2, regulatory subunit B', delta; gene trap RRG285, BayGenomics
• MGI ID: MGI:4128952
• Gene: Ppp2r5d Location: Chr17:46993917-47015952 bp, - strand Genetic Position: Chr17, 22.9 cM, cytoband C
• Alliance: Ppp2r5d^{Gt(RRG285)Byg} page

Mutation origin

• Mutant Cell Line: RRG285
• Germline Transmission: Earliest citation of germline transmission: J:161844
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The gene trap vector inserted in intron 2 and is predicted to result in a fusion transcript of exons 1 and 2 and beta-galactosidase. Western blot analysis showed about a 50% decrease in protein expression in total brain lysates from heterozygous mice. (J:161844)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRG285	CC325829 ; (MGI Seq Detail)	5' RACE	Chr17:47010996-47015836 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  13 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ppp2r5d Mutation: 34 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)