Brip1^{Gt(RRI409)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Brip1^{Gt(RRI409)Byg}
• Name: BRCA1 interacting protein C-terminal helicase 1; gene trap RRI409, BayGenomics
• MGI ID: MGI:4129733
• Synonyms: Fancj^-, Fancj^GT
• Gene: Brip1 Location: Chr11:85948964-86092019 bp, - strand Genetic Position: Chr11, 51.61 cM
• Alliance: Brip1^{Gt(RRI409)Byg} page

Mutation origin

• Mutant Cell Line: RRI409
• Germline Transmission: Earliest citation of germline transmission: J:227337
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The gene trap cassette inserted into intron 5 of the gene. This is predicted to result in a disruption of the majority of the sequence that encodes the helicase domain, loss of the BRCA1 interaction domain, and partial loss of the nuclear localization sequence. The MLH1 interaction domain, encoded within exon 5, remains intact. Western blot analysis confirmed loss of the encoded protein in MEF and testis lysates from homozygous mutant mice. (J:227115, J:227337)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRI409	CC326696 ; (MGI Seq Detail)	5' RACE	Chr11:86080560-86091984 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  3 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Brip1 Mutation: 65 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

J:227337 used the parental ES cell line E14TG2a.4.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 5 reference(s)