Wls^{Gt(RRJ545)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Wls^{Gt(RRJ545)Byg}
• Name: wntless WNT ligand secretion mediator; gene trap RRJ545, BayGenomics
• MGI ID: MGI:4129920
• Synonyms: Gpr177^lacZ, Wls^LacZ
• Gene: Wls Location: Chr3:159545309-159644300 bp, + strand Genetic Position: Chr3, 82.65 cM
• Alliance: Wls^{Gt(RRJ545)Byg} page

Mutation origin

• Mutant Cell Line: RRJ545
• Germline Transmission: Earliest citation of germline transmission: J:154679
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The beta-geo containing gene trap vector inserted between exons 9 and 10. The absence of protein expression was confirmed by immunostaining of E7.5 embryos. (J:154679)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRJ545	CC799023 ; (MGI Seq Detail)	5' RACE	Chr3:159612808-159619538 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  2 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:

33 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Wls Mutation: 38 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 8 reference(s)