Shprh^{Gt(RRJ453)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Shprh^{Gt(RRJ453)Byg}
• Name: SNF2 histone linker PHD RING helicase; gene trap RRJ453, BayGenomics
• MGI ID: MGI:4129965
• Synonyms: DELTAShprh
• Gene: Shprh Location: Chr10:11025171-11093339 bp, + strand Genetic Position: Chr10, 3.66 cM

Mutation origin

• Mutant Cell Line: RRJ453
• Germline Transmission: Unknown
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: Trapped gene association determined by genome coordinate overlap and strand. The gene trap contains a lacZ reporter gene cassette and neomycin resistance gene cassette. It inserted into intron 16, preventing the transcription of the exons coding for the RING and helicase domains. Western blot and RT-PCR experiments confirm the absence of transcription from exons 17 onward.
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRJ453	CC799073 ; (MGI Seq Detail)	5' RACE	Chr10:11046031-11057345 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  2 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Shprh Mutation: 85 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)