Sh3glb2^{Gt(OST224737)Lex}
Gene trapped Allele Detail

Summary

• Symbol: Sh3glb2^{Gt(OST224737)Lex}
• Name: SH3-domain GRB2-like endophilin B2; gene trap OST224737, Lexicon Genetics
• MGI ID: MGI:4240757
• Synonyms: B2KO
• Gene: Sh3glb2 Location: Chr2:30234821-30249349 bp, - strand Genetic Position: Chr2, 21.6 cM
• Alliance: Sh3glb2^{Gt(OST224737)Lex} page

Mutation origin

• Mutant Cell Line: OST224737
• Germline Transmission: Earliest citation of germline transmission: J:246421
• Parent Cell Line: Lex-1 (ES Cell)
• Strain of Origin: 129S5/SvEvBrd
• Project Collection: Lexicon

Mutation description

• Allele Type: Gene trapped (Null/knockout)
• Mutation: Insertion of gene trap vector
• Mutation details: A gene trap cassette inserted into intron 1 of the gene. The gene trap cassette includes a splice adaptor at the 5 end with neomycin selection and poly(A) termination signals followed by another ES cell-only promotor that expresses the first exon of the Brutons tyrosine kinase gene with multiple termination codons to prevent expression of downstream fusion transcripts. Immunoblot analysis confirmed the absence of protein expression in isolated mouse embryonic fibroblasts (MEFs) and tissues from homozygous mutant mice. (J:246421)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  OST224737	CG583007 ; (MGI Seq Detail)	3' RACE	Chr2:30244863-30245409 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  1 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sh3glb2 Mutation: 19 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)