Hpse2^{Gt(OST411605)Lex}
Gene trapped Allele Detail

Summary

• Symbol: Hpse2^{Gt(OST411605)Lex}
• Name: heparanase 2; gene trap OST411605, Lexicon Genetics
• MGI ID: MGI:4308701
• Synonyms: Hpse2^-
• Gene: Hpse2 Location: Chr19:42774978-43376794 bp, - strand Genetic Position: Chr19, 36.57 cM
• Alliance: Hpse2^{Gt(OST411605)Lex} page

Mutation origin

• Mutant Cell Line: OST411605
• Germline Transmission: Earliest citation of germline transmission: J:219597
• Parent Cell Line: Lex-1 (ES Cell)
• Strain of Origin: 129S5/SvEvBrd
• Project Collection: Lexicon

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector
• Mutation details: The gene trap vector (VICTR48) was inserted into intron 6, resulting in truncation of the carboxyl-terminus. The gene trap allele was expected to generate two separate transcripts, the marker fusion transcript (MFT) consisting of a truncated 5' region and the Neo selection marker, and the OmniBank sequence tag (OST) fusion transcript (OFT). RT-qPCR analysis revealed a ~90% reduction in 5' transcripts and nearly undetectable 3' transcripts (OFT RNA) in P14 mutant bladders. Immunoblot analysis using an antibody that recognizes an N-terminal epitope revealed only a faint signal from mutant bladder tissue. (J:219597)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  OST411605	CG651137 ; (MGI Seq Detail)	3' RACE	Chr19:42919997-42920068 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  8 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Disease models

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hpse2 Mutation: 30 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 4 reference(s)