Prpf3^{Gt(RRO284)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Prpf3^{Gt(RRO284)Byg}
• Name: pre-mRNA processing factor 3; gene trap RRO284, BayGenomics
• MGI ID: MGI:4329457
• Gene: Prpf3 Location: Chr3:95737436-95763197 bp, - strand Genetic Position: Chr3, 41.25 cM, cytoband F2
• Alliance: Prpf3^{Gt(RRO284)Byg} page

Mutation origin

• Mutant Cell Line: RRO284
• Germline Transmission: Earliest citation of germline transmission: J:141780
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT2Lxf Vector Type: gene trap
• Mutation details: The gene trap vector pGT2lxf inserted after exon 2. Southern blot analysis indicates that this insertion results in deletion of a significant portion of the gene including exons 3 - 14. Northern and western blot analysis detected about a 30% reduction in expression in retinas from heterozygous mice. (J:141780)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRO284	CG672039 ; (MGI Seq Detail)	5' RACE	Chr3:95760761-95763099 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  27 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prpf3 Mutation: 35 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141780 Graziotto JJ, et al., Decreased levels of the RNA splicing factor Prpf3 in mice and zebrafish do not cause photoreceptor degeneration. Invest Ophthalmol Vis Sci. 2008 Sep;49(9):3830-8
• All: 2 reference(s)