Prep^{Gt(RRM213)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Prep^{Gt(RRM213)Byg}
• Name: prolyl endopeptidase; gene trap RRM213, BayGenomics
• MGI ID: MGI:4329536
• Synonyms: PREP^gt
• Gene: Prep Location: Chr10:44943312-45038847 bp, + strand Genetic Position: Chr10, 23.27 cM, cytoband B2-B3
• Alliance: Prep^{Gt(RRM213)Byg} page

Mutation origin

• Mutant Cell Line: RRM213
• Germline Transmission: Earliest citation of germline transmission: J:151643
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The gene trap vector inserted into the second intron. The predicted peptide contains the first 40 amino acids of the 710 amino acid protein. The severe reduction in protein expression was confirmed by western blot analysis on brain, kidney, lung, muscle, ovary, uterus, and heart extracts. This allele is hypomorphic. (J:151643)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRM213	CG672123 ; (MGI Seq Detail)	5' RACE	Chr10:44943366-44948220 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  6 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Prep Mutation: 54 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 7 reference(s)