Eif4e^{Gt(RRO036)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Eif4e^{Gt(RRO036)Byg}
• Name: eukaryotic translation initiation factor 4E; gene trap RRO036, BayGenomics
• MGI ID: MGI:4329663
• Gene: Eif4e Location: Chr3:138231952-138265457 bp, + strand Genetic Position: Chr3, 64.3 cM
• Alliance: Eif4e^{Gt(RRO036)Byg} page

Mutation origin

• Mutant Cell Line: RRO036
• Germline Transmission: Earliest citation of germline transmission: J:224214
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT2Lxf Vector Type: gene trap
• Mutation details: The beta-geo gene trap inserted into exon 7. Western blot analysis confirmed a 50% reduction in protein expression in the kidney, heart and B cell of heterozygotes. (J:224214)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRO036	CG672254 ; (MGI Seq Detail)	5' RACE	multiple
  * GRCm39; (strand of gene trap mutagenesis)

Expression

In Mice Carrying this Mutation:

37 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Eif4e Mutation: 42 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 5 reference(s)