Sptan1^{Gt(RRQ171)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Sptan1^{Gt(RRQ171)Byg}
• Name: spectrin alpha, non-erythrocytic 1; gene trap RRQ171, BayGenomics
• MGI ID: MGI:4330132
• Gene: Sptan1 Location: Chr2:29855572-29921463 bp, + strand Genetic Position: Chr2, 20.93 cM, cytoband B
• Alliance: Sptan1^{Gt(RRQ171)Byg} page

Mutation origin

• Mutant Cell Line: RRQ171
• Germline Transmission: Earliest citation of germline transmission: J:186305
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: The gene trap vector inserted into intron 24. Western blot analysis with alpha2-C antibodies confirmed the absence of alpha 2 spectrin protein expression. (J:186305)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRQ171	CG784290 ; (MGI Seq Detail)	5' RACE	Chr2:29890012-29893427 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  59 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sptan1 Mutation: 130 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 4 reference(s)