Vrk1^{Gt(RRR178)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Vrk1^{Gt(RRR178)Byg}
• Name: vaccinia related kinase 1; gene trap RRR178, BayGenomics
• MGI ID: MGI:4330710
• Synonyms: GT3, Vrk1^Gt
• Gene: Vrk1 Location: Chr12:105976487-106043685 bp, + strand Genetic Position: Chr12, 56.37 cM, cytoband F1
• Alliance: Vrk1^{Gt(RRR178)Byg} page

Mutation origin

• Mutant Cell Line: RRR178
• Germline Transmission: Earliest citation of germline transmission: J:156653
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Hypomorph, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: Insertion of a beta-gal and neo cassette within 6174 bases 3' of the beginning of intron 3 of Vrk1. The predicted product only contains the N-terminal 72 amino acids fused with beta-gal and is catalytically inert. However, RT-PCR demonstrated only a 4.5- to 6.4-fold decrease in full length message in various adult tissues, confirmed by immunoblots, indicating this is a hypomorphic allele. (J:156653)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRR178	CG784892 ; (MGI Seq Detail)	5' RACE	Chr12:105976519-106009149 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  39 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Vrk1 Mutation: 42 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 7 reference(s)