Ccnt1^{Gt(RRR115)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Ccnt1^{Gt(RRR115)Byg}
• Name: cyclin T1; gene trap RRR115, BayGenomics
• MGI ID: MGI:4330764
• Synonyms: CycT1^-
• Gene: Ccnt1 Location: Chr15:98436570-98468340 bp, - strand Genetic Position: Chr15, 54.54 cM, cytoband F2
• Alliance: Ccnt1^{Gt(RRR115)Byg} page

Mutation origin

• Mutant Cell Line: RRR115
• Germline Transmission: Earliest citation of germline transmission: J:130071
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Hypomorph, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The gene trap vector inserted into intron 3 of the gene. The insertion of the trap sequence introduces a splice acceptor, a stop codon and a strong polyadenylation site, thus splicing nascent transcripts of the Ccnt1 gene to the trap to create a fusion protein between CCNT1 and beta-geo, which contains only 18% of CCNT1 amino acid sequence. Western blot analysis of cell lysates from various organs demonstrated that, when normalized to levels of total protein, homozygous mutant mice exhibit some residual (less than 5%) expression of CCNT1 protein relative to wild-type controls. (J:130071)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRR115	CG784948 ; (MGI Seq Detail)	5' RACE	Chr15:98458079-98465607 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  14 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ccnt1 Mutation: 41 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)