Mcm3^{Gt(RRR002)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Mcm3^{Gt(RRR002)Byg}
• Name: minichromosome maintenance complex component 3; gene trap RRR002, BayGenomics
• MGI ID: MGI:4330835
• Synonyms: Mcm3^Gt
• Gene: Mcm3 Location: Chr1:20873192-20890536 bp, - strand Genetic Position: Chr1, 6.5 cM, cytoband A3-A5
• Alliance: Mcm3^{Gt(RRR002)Byg} page

Mutation origin

• Mutant Cell Line: RRR002
• Germline Transmission: Earliest citation of germline transmission: J:165667
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The gene trap vector inserted between exon 6 and 7. Quantitative RT-PCR confirmed a 50% decrease in transcript expression in heterozygous mouse embryonic fibroblasts. (J:165667)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRR002	CG785023 ; (MGI Seq Detail)	5' RACE	Chr1:20884624-20887614 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  7 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mcm3 Mutation: 43 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 6 reference(s)