Kmt2d^{Gt(RRT024)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Kmt2d^{Gt(RRT024)Byg}
• Name: lysine (K)-specific methyltransferase 2D; gene trap RRT024, BayGenomics
• MGI ID: MGI:4332076
• Synonyms: Kmt2d^betaGeo, Mll2^{Gt(RRt024)Byg}, MLL4^-
• Gene: Kmt2d Location: Chr15:98729550-98769085 bp, - strand
• Alliance: Kmt2d^{Gt(RRT024)Byg} page

Mutation origin

• Mutant Cell Line: RRT024
• Germline Transmission: Earliest citation of germline transmission: J:225779
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: Trapped gene association determined by genome coordinate overlap and strand. An expression cassette encoding a beta-galactosidase neomycin resistance fusion protein inserted into intron 50. Inclusion of a splice acceptor sequence and a 3' end cleavage and polyadenylation signal at the 5' and 3' ends of the beta-Geo cassette, respectively, is predicted to generated a truncated protein with peptide sequence corresponding to the first 50 exons fused to beta-Geo but lacking the SET domain and methyltransferase activity. Real-time PCR confirmed a 50% reduction of mRNA containing exon 52 in heterozygotes. (J:225779)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRT024	CG887042 ; (MGI Seq Detail)	5' RACE	Chr15:98735176-98737310 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  17 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kmt2d Mutation: 169 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 12 reference(s)