Appl1^{Gt(RRS819)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Appl1^{Gt(RRS819)Byg}
• Name: adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1; gene trap RRS819, BayGenomics
• MGI ID: MGI:4332126
• Gene: Appl1 Location: Chr14:26640943-26692567 bp, - strand Genetic Position: Chr14, 16.09 cM, cytoband B
• Alliance: Appl1^{Gt(RRS819)Byg} page

Mutation origin

• Mutant Cell Line: RRS819
• Germline Transmission: Earliest citation of germline transmission: J:211794
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The gene trap vector, pGTxf, which contains a splice acceptor-beta geo cassette-polyadenylation sequence, was inserted between exon 7 and exon 8. The insertion results in the disruption of the gene transcription and expression. Western blot analysis confirmed absence of protein expression in liver, fat, and skeletal muscle tissue homogenates from homozygous mutant mice. (J:211794)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRS819	CG887093 ; (MGI Seq Detail)	5' RACE	Chr14:26680414-26681424 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  8 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Appl1 Mutation: 47 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)