Tdp2^{Gt(RRS512)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Tdp2^{Gt(RRS512)Byg}
• Name: tyrosyl-DNA phosphodiesterase 2; gene trap RRS512, BayGenomics
• MGI ID: MGI:4332298
• Synonyms: Ttrap^gt
• Gene: Tdp2 Location: Chr13:25015662-25026136 bp, + strand Genetic Position: Chr13, 10.7 cM
• Alliance: Tdp2^{Gt(RRS512)Byg} page

Mutation origin

• Mutant Cell Line: RRS512
• Germline Transmission: Earliest citation of germline transmission: J:153265
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutations: Duplication, Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Tdp2^{Gt(RRS512)Byg} involves 1 genes/genome features (Tdp2) View all
• Mutation details: The trapping vector pGT0lxf was inserted into intron 5. Quantitative PCR and FISH analysis indicated that more than 40 copies of the vector had integrated in a single site. No beta-galactosidase staining was detected during embryogenesis; however, lacZ mRNA expression is detected. RT-PCR analysis detected the presence of wild-type mRNA in homozygous trapped mice. Further analysis revealed a partial duplication of the wild-type gene including exons 1 - 5 that appeared to be inserted after the tandem vector insertion.
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRS512	CG887267 ; (MGI Seq Detail)	5' RACE	Chr13:25020123-25020934 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  3 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:

6 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tdp2 Mutation: 25 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)