Casc3^{Gt(RRU345)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Casc3^{Gt(RRU345)Byg}
• Name: exon junction complex subunit; gene trap RRU345, BayGenomics
• MGI ID: MGI:4338383
• Synonyms: Casc3^RRU345
• Gene: Casc3 Location: Chr11:98700634-98724633 bp, + strand Genetic Position: Chr11, 62.68 cM
• Alliance: Casc3^{Gt(RRU345)Byg} page

Mutation origin

• Mutant Cell Line: RRU345
• Germline Transmission: Earliest citation of germline transmission: J:240595
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Hypomorph)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: A gene trap vector inserted into the intron between exons 3 and 4. The resulting insertional mutation is predicted to constitutively produce a fusion transcript comprised of exons 1-3 of the endogenous gene with the beta-galactosidase (LacZ) gene. Compared to controls, heterozygous mutants showed a 52%-54% whereas homozygous mutants showed a 90%-95% reduction in wild-type mRNA levels. Western analyses of dorsal cortical lysates using CASC3 antibody showed that a single protein product was detected. Compared to controls, wild-type expressed protein level was reduced by 72% in E16.5 homozygous mutant cortices, which was also apparent at E11.5 and E15.5. This suggests that this mutation is a strong hypomorphic allele. (J:240595)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRU345	CL265285 ; (MGI Seq Detail)	5' RACE	Chr11:98700888-98701762 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  5 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:

48 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Casc3 Mutation: 41 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)