Daam1^{Gt(RRT390)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Daam1^{Gt(RRT390)Byg}
• Name: dishevelled associated activator of morphogenesis 1; gene trap RRT390, BayGenomics
• MGI ID: MGI:4338561
• Synonyms: Daam1^gt
• Gene: Daam1 Location: Chr12:71877852-72039107 bp, + strand Genetic Position: Chr12, 30.06 cM, cytoband C3
• Alliance: Daam1^{Gt(RRT390)Byg} page

Mutation origin

• Mutant Cell Line: RRT390
• Germline Transmission: Earliest citation of germline transmission: J:167714
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Hypomorph, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: A floxed gene trap cassette inserted into intron 3 of the gene. Rt-PCR and western blot analysis revealed the expression of a fusion between the first 3 exons of the gene and the Beta-geo reporter. In addition, low levels of expression of the wild-type transcript and protein were detected indicating that this is a hypomorphic allele. (J:167714)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRT390	CL265475 ; (MGI Seq Detail)	5' RACE	Chr12:71877904-71962559 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  19 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Daam1 Mutation: 77 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 6 reference(s)