Brd2^{Gt(RRT234)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Brd2^{Gt(RRT234)Byg}
• Name: bromodomain containing 2; gene trap RRT234, BayGenomics
• MGI ID: MGI:4340588
• Gene: Brd2 Location: Chr17:34330993-34341581 bp, - strand Genetic Position: Chr17, 17.98 cM, cytoband D
• Alliance: Brd2^{Gt(RRT234)Byg} page

Mutation origin

• Mutant Cell Line: RRT234
• Germline Transmission: Earliest citation of germline transmission: J:159964
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The gene trap vector inserted into intron 1 upstream of exon 2, which contains the first translation initiation site. The reduction in protein expression was confirmed by western blot analysis on embryonic stem cell extracts.
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRT234	CL315277 ; (MGI Seq Detail)	5' RACE	Chr17:34338744-34338794 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  15 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Brd2 Mutation: 45 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)