Stk38l^{Gt(RRT116)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Stk38l^{Gt(RRT116)Byg}
• Name: serine/threonine kinase 38 like; gene trap RRT116, BayGenomics
• MGI ID: MGI:4340648
• Synonyms: Ndr2^-
• Gene: Stk38l Location: Chr6:146626493-146680310 bp, + strand Genetic Position: Chr6, 77.7 cM
• Alliance: Stk38l^{Gt(RRT116)Byg} page

Mutation origin

• Mutant Cell Line: RRT116
• Germline Transmission: Earliest citation of germline transmission: J:208108
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: Trapped gene association determined by genome coordinate overlap and strand. The position of the gene trap was identified with a 5' rapid amplification of cDNA ends (5'RACE) sequence tag matching exons 9-7 of the gene and thus indicating the gene trap location in intron 9. Serial PCR and sequence analyses showed that the gene trap vector integrated at position bp 995 of intron 9 of the gene. Western blot analysis confirmed absence of full-length protein in the hippocampus and the presence of a Stk38l(1-282)::beta-geo fusion protein that has most of the activating sequence, the kinase subdomains VIII-XII, including the substrate recognition sites and the c-terminal hydrophobic motif deleted. (J:208108)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRT116	CL315338 ; (MGI Seq Detail)	5' RACE	Chr6:146669912-146670652 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  20 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Stk38l Mutation: 37 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 4 reference(s)