Grhl2^{Gt(RRU622)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Grhl2^{Gt(RRU622)Byg}
• Name: grainyhead like transcription factor 2; gene trap RRU622, BayGenomics
• MGI ID: MGI:4340678
• Synonyms: Grhl2^LacZ4
• Gene: Grhl2 Location: Chr15:37233280-37363813 bp, + strand Genetic Position: Chr15, 14.84 cM, cytoband C
• Alliance: Grhl2^{Gt(RRU622)Byg} page

Mutation origin

• Mutant Cell Line: RRU622
• Germline Transmission: Earliest citation of germline transmission: J:167065
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: Trapped gene association determined by genome coordinate overlap and strand. The gene trap vector was inserted at the beginning of intron 4. No mRNA could be detected in homozygous embryo extracts by RT-PCR. Antibody to the N-terminal region of the protein showed cytoplasmic staining unlike the nuclear staining seen in wild type embryos. (J:167065)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRU622	CL315368 ; (MGI Seq Detail)	5' RACE	Chr15:37279605-37279968 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  46 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Grhl2 Mutation: 41 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 5 reference(s)