Pcnt^{Gt(RRU388)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Pcnt^{Gt(RRU388)Byg}
• Name: pericentrin (kendrin); gene trap RRU388, BayGenomics
• MGI ID: MGI:4340776
• Gene: Pcnt Location: Chr10:76187097-76278620 bp, - strand Genetic Position: Chr10, 38.8 cM
• Alliance: Pcnt^{Gt(RRU388)Byg} page

Mutation origin

• Mutant Cell Line: RRU388
• Germline Transmission: Earliest citation of germline transmission: J:226309
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: A gene trap vector containing a splice-acceptor sequence upstream of the reporter gene, beta-geo (a fusion gene of beta-galactosidase and neomycin phosphotransferase II) inserted into intron 1. Immunoblot analysis showed a complete loss of the encoded protein in the lysates from whole embryos and immortalized MEFs. (J:226309)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRU388	CL315467 ; (MGI Seq Detail)	5' RACE	Chr10:76278400-76278530 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  52 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Disease models

Expression

In Structures Affected by this Mutation:

23 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pcnt Mutation: 159 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)