Sec23b^{Gt(AD0407)Wtsi}
Gene trapped Allele Detail

Summary

• Symbol: Sec23b^{Gt(AD0407)Wtsi}
• Name: SEC23 homolog B, COPII coat complex component; gene trap AD0407, Wellcome Trust Sanger Institute
• MGI ID: MGI:4345347
• Synonyms: Sec23b^gt
• Gene: Sec23b Location: Chr2:144398165-144432673 bp, + strand Genetic Position: Chr2, 71.02 cM, cytoband H1
• Alliance: Sec23b^{Gt(AD0407)Wtsi} page

Small size of Sec23b^{Gt(AD0407)Wtsi}/Sec23b^{Gt(AD0407)Wtsi} neonates

Show the 7 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: AD0407
• Germline Transmission: Earliest citation of germline transmission: J:186391
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Sanger Inst. Gene Trap Res.

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0lxr
• Mutation details: The gene trap vector inserted into intron 19. RT-PCR analysis indicated a more than 400-fold reduction in expression of wild-type transcript in homozygous mice. Immunoblot analysis failed to detect wild-type protein expression in MEFs from homozygous mice. The fusion protein consisting of the first 19 exons fused to the beta-geo reporter is detected in heterozygous and homozygous cells. The fusion protein is coimmunoprecipitated with SEC24A in MEF lysates. (J:186391)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  AD0407	CL525003 ; (MGI Seq Detail)	5' RACE	Chr2:144428776-144429486 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  19 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Generation of the Sec23b^{Gt(AD0407)Wtsi} allele

Expression

In Structures Affected by this Mutation:

14 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sec23b Mutation: 50 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 5 reference(s)