Rnf8^{Gt(AS0574)Wtsi}
Gene trapped Allele Detail

Summary

• Symbol: Rnf8^{Gt(AS0574)Wtsi}
• Name: ring finger protein 8; gene trap AS0574, Wellcome Trust Sanger Institute
• MGI ID: MGI:4346727
• Synonyms: Rnf8^-
• Gene: Rnf8 Location: Chr17:29833763-29860638 bp, + strand Genetic Position: Chr17, 15.45 cM
• Alliance: Rnf8^{Gt(AS0574)Wtsi} page

Impaired spermatogenesis in Rnf8^{Gt(AS0574)Wtsi}/Rnf8^{Gt(AS0574)Wtsi} and Rnf8^{Gt(RRR260)Byg}/Rnf8^{Gt(RRR260)Byg} males

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: AS0574
• Germline Transmission: Earliest citation of germline transmission: J:161240
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Sanger Inst. Gene Trap Res.

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0lxr
• Mutation details: The beta-geo cassette inserted between exon 2 and 3. The absence of protein expression was confirmed by western blot analysis on thymocyte extracts. (J:161240)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  AS0574	CL570006 ; (MGI Seq Detail)	5' RACE	Chr17:29838454-29840610 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  3 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Generation of the Rnf8^{Gt(AS0574)Wtsi} and Rnf8^{Gt(RRR260)Byg} alleles

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Rnf8^{Gt(AS0574)Wtsi}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rnf8 Mutation: 21 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 5 reference(s)