Myc^tm4.1Fwa
Targeted Allele Detail

Summary

• Symbol: Myc^tm4.1Fwa
• Name: myelocytomatosis oncogene; targeted mutation 4.1, Frederick W Alt
• MGI ID: MGI:4352684
• Synonyms: c-Myc^25Isce
• Gene: Myc Location: Chr15:61857240-61862223 bp, + strand Genetic Position: Chr15, 26.19 cM, cytoband D2-D3
• Alliance: Myc^tm4.1Fwa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:150339
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Insertion
• Mutation details: A cassette containing 25 tandem I-SceI target sites (S. cerevisiae) endonuclease recognition site (TAGGGATAACAGGGTAAT) was inserted into intron 1 of the gene. A floxed neomycin cassette was included upstream of the I-SceI cassette and was subsequently removed by transient expression of cre recombinase. Correct targeting was confirmed by Southern blotting. (J:150339)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Myc Mutation: 43 strains or lines available

References

• Original: J:150339 Wang JH, et al., Mechanisms promoting translocations in editing and switching peripheral B cells. Nature. 2009 Jul 9;460(7252):231-6
• All: 6 reference(s)