Antxr1^tm1.2Lepp
Targeted Allele Detail

Summary

• Symbol: Antxr1^tm1.2Lepp
• Name: anthrax toxin receptor 1; targeted mutation 1.2, Stephen H Leppla
• MGI ID: MGI:4352941
• Synonyms: TEM8^-
• Gene: Antxr1 Location: Chr6:87110835-87312757 bp, - strand Genetic Position: Chr6, 37.94 cM
• Alliance: Antxr1^tm1.2Lepp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:150952
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 13, which encodes the transmembrane domain, and an frt flanked neo cassette and second loxP site were inserted downstream of exon 13. Cre mediated recombination then removed exon 13 l and (when present) the neo cassette leaving behind a single loxP site. Western blot analysis confirmed the absence of protein expression in lysates from homozygous MEFs but a mutant protein was detected in conditioned media from homozygous MEFs. (J:150952)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Antxr1 Mutation: 30 strains or lines available

References

• Original: J:150952 Liu S, et al., Capillary morphogenesis protein-2 is the major receptor mediating lethality of anthrax toxin in vivo. Proc Natl Acad Sci U S A. 2009 Jul 28;106(30):12424-9
• All: 6 reference(s)