Pax7^{tm2.1(cre/ERT2)Fan}
Targeted Allele Detail

Summary

• Symbol: Pax7^{tm2.1(cre/ERT2)Fan}
• Name: paired box 7; targeted mutation 2.1, Chen-Ming Fan
• MGI ID: MGI:4353154
• Synonyms: Pax7^CE, Pax7^Cre-ERT2
• Gene: Pax7 Location: Chr4:139464373-139560839 bp, - strand Genetic Position: Chr4, 70.83 cM
• Alliance: Pax7^{tm2.1(cre/ERT2)Fan} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:150962
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inducible, Null/knockout, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Pax7^{tm2.1(cre/ERT2)Fan} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Pax7 (MGI:97491)

• Mutation details: Beginning with the Pax7^tm1.2Sjc allele in which exon 2 was knocked out leaving a single loxP site, part of exon 1 was replaced with a cre/ERT2 (ESR1) fusion followed by an IRES-DsRed and an frt flanked neo cassette. Germ line, flp mediated recombination removed the neo cassette. The absence of full-length Pax7 protein expression was confirmed by western blot analysis on P10 tibialis anterior muscle extracts. Expression of the fusion cre/ERT2 protein was confirmed by a cross with a reporter line. DsRed fluorescence was not detectable by epifluorescence nor was DsRed protein by immunostaining. (J:150962)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Pax7 (mouse) Summary of all recombinase alleles driven by Pax7. Comparative matrix view of recombinase activities Alliance mouse Pax7 gene page

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pax7 Mutation: 38 strains or lines available

References

• Original: J:150962 Lepper C, et al., Adult satellite cells and embryonic muscle progenitors have distinct genetic requirements. Nature. 2009 Jul 30;460(7255):627-31
• All: 109 reference(s)