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Pax7tm2.1(cre/ERT2)Fan
Targeted Allele Detail
Summary
Symbol: Pax7tm2.1(cre/ERT2)Fan
Name: paired box 7; targeted mutation 2.1, Chen-Ming Fan
MGI ID: MGI:4353154
Synonyms: Pax7CE, Pax7Cre-ERT2
Gene: Pax7  Location: Chr4:139464373-139560839 bp, - strand  Genetic Position: Chr4, 70.83 cM
Alliance: Pax7tm2.1(cre/ERT2)Fan page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:150962
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Inducible, Null/knockout, Recombinase)
Inducer:    tamoxifen
Mutations:    Insertion, Intragenic deletion
 
Pax7tm2.1(cre/ERT2)Fan expression driven by 1 gene
 
Mutation detailsBeginning with the Pax7tm1.2Sjc allele in which exon 2 was knocked out leaving a single loxP site, part of exon 1 was replaced with a cre/ERT2 (ESR1) fusion followed by an IRES-DsRed and an frt flanked neo cassette. Germ line, flp mediated recombination removed the neo cassette. The absence of full-length Pax7 protein expression was confirmed by western blot analysis on P10 tibialis anterior muscle extracts. Expression of the fusion cre/ERT2 protein was confirmed by a cross with a reporter line. DsRed fluorescence was not detectable by epifluorescence nor was DsRed protein by immunostaining. (J:150962)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Pax7 (mouse)
Summary of all recombinase alleles driven by Pax7.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 2 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Pax7 Mutation:  38 strains or lines available
References
Original:  J:150962 Lepper C, et al., Adult satellite cells and embryonic muscle progenitors have distinct genetic requirements. Nature. 2009 Jul 30;460(7255):627-31
All:  109 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory