Gt(ROSA)26Sor^{tm8(RNAi:Crhr1)Rkuhn}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm8(RNAi:Crhr1)Rkuhn}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 8, Ralf Kuhn
• MGI ID: MGI:4353628
• Synonyms: shCRHR1
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm8(RNAi:Crhr1)Rkuhn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151155
• Parent Cell Line: IDG3.2 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm8(RNAi:Crhr1)Rkuhn} involves 1 genes/genome features (Crhr1) View all
• Mutation details: Using RMCE, an inserted hygro cassette was replaced with a neo cassette and a short hairpin RNA driven by the human U6 promoter that targets Crhr1. Expression of the shRNA results in an 80% reduction in endogenous Crhr1 transcript levels compared to in wild-type mice. (J:151155)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 992 strains or lines available

References

• Original: J:151155 Delic S, et al., Genetic mouse models for behavioral analysis through transgenic RNAi technology. Genes Brain Behav. 2008 Oct;7(7):821-30
• All: 1 reference(s)