Tg(Tyr-cre/ERT,-Hras1*,-Trap1a)10BJvde
Transgene Detail

Summary

• Symbol: Tg(Tyr-cre/ERT,-Hras1*,-Trap1a)10BJvde
• Name: transgene insertion 10B, Benoit Van den Eynde
• MGI ID: MGI:4354013
• Synonyms: Tg(Tyr-cre/ESR1*,-Hras1*,-Trap1a)10BJvde, TiRP-10B
• Transgene: Tg(Tyr-cre/ERT,-Hras1*,-Trap1a)10BJvde Location: unknown
• Alliance: Tg(Tyr-cre/ERT,-Hras1*,-Trap1a)10BJvde page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Conditional ready, Inducible, Inserted expressed sequence, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Tyr-cre/ERT,-Hras1*,-Trap1a)10BJvde expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Hras (MGI:96224)
  mouse	Trap1a (MGI:98818)

• Tg(Tyr-cre/ERT,-Hras1*,-Trap1a)10BJvde expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Tyr (MGI:98880)

• Mutation details: The mouse promoter was used to drive tamoxifen-inducible, melanocyte-specific expression of a floxed cre/ESR1 fusion containing the aminoacid substitution G521R followed out of frame by a constitutively active Hras1 with the G12V amino acid substitution, an IRES, and the mouse cDNA for Trap1a with its polyadenylation signal. Removal of the floxed cre/ERT fusion is required to express downstream genes. Eight founders were generated (TiRP-1-5, TiRP-5-6, TiRP-8/9A, TiRP-8/9B, TiRP-10A, TiRP-10B, TiRP-14 , and TiRP-16) and each contained concatemers of the transgene in head to tail or tail to tail orientations. Cre mediated recombination removes all but one copy of the recombined transgene. Activity for this line is greater in melanocytes in the hair bulbs and is not seen in the brain, eye, spleen, thymus, kidney, liver, heart, or lung. (J:107175)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tyr (mouse) Summary of all recombinase alleles driven by Tyr. Comparative matrix view of recombinase activities Alliance mouse Tyr gene page

Disease models

Tumor Data

List all tumor models in MMHCdb carrying Tg(Tyr-cre/ERT,-Hras1*,-Trap1a)10BJvde

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:107175 Huijbers IJ, et al., An inducible mouse model of melanoma expressing a defined tumor antigen. Cancer Res. 2006 Mar 15;66(6):3278-86
• All: 6 reference(s)