Rb1^tm2.1Jyjw
Targeted Allele Detail

Summary

• Symbol: Rb1^tm2.1Jyjw
• Name: RB transcriptional corepressor 1; targeted mutation 2.1, Jean YJ Wang
• MGI ID: MGI:4354271
• Synonyms: Rb1^N750F, Rb1^NF
• Gene: Rb1 Location: Chr14:73430298-73563446 bp, - strand Genetic Position: Chr14, 38.73 cM, cytoband D3
• Alliance: Rb1^tm2.1Jyjw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151417
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A targeting vector targeted to exon 22 was used to substitute a bulky phenylalanine for asparagine at amino acid 750, which is predicted to sterically block access to the LXCXE binding cleft. Southern blotting confirmed successful recombination. Analysis of mutant fibroblasts confirmed that protein levels were equivalent to wild-type cells. Crossing with mice expressing cre recombinase in the germline removed a floxed neo included in the vector. (J:151417)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rb1 Mutation: 111 strains or lines available

References

• Original: J:151417 Francis SM, et al., A functional connection between pRB and transforming growth factor beta in growth inhibition and mammary gland development. Mol Cell Biol. 2009 Aug;29(16):4455-66
• All: 2 reference(s)