Gt(ROSA)26Sor^{tm31(H1/tetO-RNAi:Insr)Arte}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm31(H1/tetO-RNAi:Insr)Arte}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 31, TaconicArtemis
• MGI ID: MGI:4355015
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm31(H1/tetO-RNAi:Insr)Arte} page

Mutation origin

• Mutant Cell Line: B6-RMCE 68
• Germline Transmission: Earliest citation of germline transmission: J:151686
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Inducible, Knockdown, RMCE-ready)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm31(H1/tetO-RNAi:Insr)Arte} involves 1 genes/genome features (Insr) View all
• Mutation details: A short hairpin RNA directed against the insulin receptor (Insr) gene was targeted into the Rosa26 locus. The shRNA sequence is under the regulation of the tetO operator and a codon optimized tet repressor (itetR) under the control of a CAG promoter. The construct was transfected into Art B6-RMCE 68 ES cells derived from the C57BL/6 strain through Recombinase Mediated Cassette Exchange. (J:151686)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

Notes

Parent cell line was derived from C57BL/6

References

• Original: J:151686 Seibler J, et al., Reversible gene knockdown in mice using a tight, inducible shRNA expression system. Nucleic Acids Res. 2007;35(7):e54
• All: 2 reference(s)