Gad2^{tm1(cre/ERT2)Zjh}
Targeted Allele Detail

Summary

• Symbol: Gad2^{tm1(cre/ERT2)Zjh}
• Name: glutamic acid decarboxylase 2; targeted mutation 1, Z Josh Huang
• MGI ID: MGI:4355159
• Synonyms: Gad2-CreER, Gad2-CreERT2, GAD65-CreER, GAD65-CreERT2
• Gene: Gad2 Location: Chr2:22512262-22583889 bp, + strand Genetic Position: Chr2, 15.15 cM, cytoband A-B
• Alliance: Gad2^{tm1(cre/ERT2)Zjh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151755
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1
• Project Collection: Neuroscience Blueprint cre

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Gad2^{tm1(cre/ERT2)Zjh} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gad2 (MGI:95634)

• Mutation details: A targeting vector was designed to insert a CreERT2 fusion gene (Cre-ERT2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), an SV40 polyA signal, and an frt-flanked neo cassette into the initiation codon of the Gad2 (glutamic acid decarboxylase 2) gene. This construct was electroporated into 129 x C57BL/6 hybrid embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with white C57BL/6 mice to originate the colony. Mutant mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background (N10)) to remove the neo selection cassette. (J:151755)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gad2 (mouse) Summary of all recombinase alleles driven by Gad2. Comparative matrix view of recombinase activities Alliance mouse Gad2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gad2 Mutation: 60 strains or lines available

Notes

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References

• Original: J:151755 NIH Neuroscience Blueprint Cre Driver Network, Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network. MGI Direct Data Submission. 2009
• All: 16 reference(s)