Ifnb1tm2.1Lien
Targeted Allele Detail
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Symbol: |
Ifnb1tm2.1Lien |
Name: |
interferon beta 1, fibroblast; targeted mutation 2.1, Stefan Lienenklaus |
MGI ID: |
MGI:4355683 |
Synonyms: |
IFN-Bflox beta-luc, IFN-Bfloxbeta-luc |
Gene: |
Ifnb1 Location: Chr4:88440262-88441011 bp, - strand Genetic Position: Chr4, 41.91 cM
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Alliance: |
Ifnb1tm2.1Lien page
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Germline Transmission: |
Earliest citation of germline transmission:
J:151854
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Parent Cell Line: |
Bruce 4 (ES Cell)
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Strain of Origin: |
B6.Cg-Thy1a
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Allele Type: |
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Targeted (Conditional ready, No functional change) |
Mutation: |
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Insertion
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Mutation details: A loxP site was inserted 42 bp upstream of the lone coding exon and a Frt-flanked neomycin cassette, another loxP site, and a myc-tagged luciferase coding sequence were inserted downstream of the exon in the BstAPI restriction site. After targeting in C57BL/6 ES cells, the neomycin cassette was removed by crossing founder mice with FLP-deleter mice. A poly-A sequence upstream of the luciferase cassette prevents transcription of the reporter until the endogenous exon is floxed.
(J:151854)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Ifnb1 Mutation: |
21 strains or lines available
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Original: |
J:151854 Lienenklaus S, et al., Novel reporter mouse reveals constitutive and inflammatory expression of IFN-beta in vivo. J Immunol. 2009 Sep 1;183(5):3229-36 |
All: |
5 reference(s) |
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