Klrk1^tm1.1Bpol
Targeted Allele Detail

Summary

• Symbol: Klrk1^tm1.1Bpol
• Name: killer cell lectin-like receptor subfamily K, member 1; targeted mutation 1.1, Bojan Polic
• MGI ID: MGI:4355860
• Synonyms: Klrk1^-, NKG2D^-
• Gene: Klrk1 Location: Chr6:129587286-129600827 bp, - strand Genetic Position: Chr6, 63.44 cM
• Alliance: Klrk1^tm1.1Bpol page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151878
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The locus contains two alternative start sites, one in exon 2 and one in exon 3. The ATG start site in exon 2 was mutated into an EcoRI restriction site. Exon 3 was replaced with a cassette containing an EGFP coding sequence, a transcriptional stop signal, and a loxP-flanked neomycin resistance cassette. Correctly targeted ES cells had the neomycin cassette removed by transient expression of cre recombinase. Heterozygote natural killer (NK) cells had reduced protein expression of NKG2D while no protein expression was detected on homozygote NK cells as determined by flow cytometry. EGFP expression was not detected and subsequent sequence analysis of the targeting vector revealed the Kozak sequence at the leading ATG of the EGFP coding sequence was deleted during sub-cloning. (J:151878, J:215590)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Klrk1 Mutation: 27 strains or lines available

References

• Original: J:151878 Zafirova B, et al., Altered NK cell development and enhanced NK cell-mediated resistance to mouse cytomegalovirus in NKG2D-deficient mice. Immunity. 2009 Aug 21;31(2):270-82
• All: 20 reference(s)