Cd1d1tm1.1Boes
Targeted Allele Detail
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Symbol: |
Cd1d1tm1.1Boes |
Name: |
CD1d1 antigen; targeted mutation 1.1, Marianne Boes |
MGI ID: |
MGI:4355877 |
Synonyms: |
Cd1d-EYFP |
Gene: |
Cd1d1 Location: Chr3:86903141-86906748 bp, - strand Genetic Position: Chr3, 37.91 cM
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Alliance: |
Cd1d1tm1.1Boes page
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Germline Transmission: |
Earliest citation of germline transmission:
J:151699
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Parent Cell Line: |
Bruce 4 (ES Cell)
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Strain of Origin: |
B6.Cg-Thy1a
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Allele Type: |
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Targeted (Null/knockout, Reporter) |
Mutation: |
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Insertion
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Mutation details: An EYFP expression cassette and a floxed neomycin resistance cassette were inserted in-frame downstream of the last coding exon but upstream of the 3' untranslated region. Founder mice were crossed with cre-deleter mice to remove the neomycin cassette, leaving behind a loxP site. Immunoblot analysis of homozygote thymocyte extracts detected expression of just the fusion product. The fusion product was also detectable by flow cytometry. Developmental defects in a subset of homozygote NK T cells suggest some function of the endogenous protein is lost.
(J:151699)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Cd1d1 Mutation: |
34 strains or lines available
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Original: |
J:151699 Sille FC, et al., Distinct requirements for CD1d intracellular transport for development of V(alpha)14 iNKT cells. J Immunol. 2009 Aug 1;183(3):1780-8 |
All: |
1 reference(s) |
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