Cd1d1^tm1.1Boes
Targeted Allele Detail

Summary

• Symbol: Cd1d1^tm1.1Boes
• Name: CD1d1 antigen; targeted mutation 1.1, Marianne Boes
• MGI ID: MGI:4355877
• Synonyms: Cd1d-EYFP
• Gene: Cd1d1 Location: Chr3:86903141-86906748 bp, - strand Genetic Position: Chr3, 37.91 cM
• Alliance: Cd1d1^tm1.1Boes page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151699
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: An EYFP expression cassette and a floxed neomycin resistance cassette were inserted in-frame downstream of the last coding exon but upstream of the 3' untranslated region. Founder mice were crossed with cre-deleter mice to remove the neomycin cassette, leaving behind a loxP site. Immunoblot analysis of homozygote thymocyte extracts detected expression of just the fusion product. The fusion product was also detectable by flow cytometry. Developmental defects in a subset of homozygote NK T cells suggest some function of the endogenous protein is lost. (J:151699)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd1d1 Mutation: 35 strains or lines available

References

• Original: J:151699 Sille FC, et al., Distinct requirements for CD1d intracellular transport for development of V(alpha)14 iNKT cells. J Immunol. 2009 Aug 1;183(3):1780-8
• All: 1 reference(s)