Prdm1^tm2.1Nutt
Targeted Allele Detail

Summary

• Symbol: Prdm1^tm2.1Nutt
• Name: PR domain containing 1, with ZNF domain; targeted mutation 2.1, Stephen L Nutt
• MGI ID: MGI:4355900
• Synonyms: Blimp1^fl
• Gene: Prdm1 Location: Chr10:44313173-44404497 bp, - strand Genetic Position: Chr10, 23.24 cM
• Alliance: Prdm1^tm2.1Nutt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151858
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted upstream of exon 6 with an additional loxP site and a frt-flanked neomycin/EGFP cassette inserted downstream of exon 6. The neomycin/EGFP cassette was subsequently removed by crossing founder mice with a flpe transgenic mice, leaving exon 6 floxed. Removal of exon 6 by cre-mediated recombination will leave exon 7 out-of-frame and create a null allele. (J:151858)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Prdm1 Mutation: 66 strains or lines available

References

• Original: J:151858 Kallies A, et al., Blimp-1 transcription factor is required for the differentiation of effector CD8(+) T cells and memory responses. Immunity. 2009 Aug 21;31(2):283-95
• All: 17 reference(s)