Ccl2^tm1Tyos
Targeted Allele Detail

Summary

• Symbol: Ccl2^tm1Tyos
• Name: C-C motif chemokine ligand 2; targeted mutation 1, Teizo Yoshimura
• MGI ID: MGI:4355945
• Synonyms: MCP-1^flox-neo
• Gene: Ccl2 Location: Chr11:81926403-81928278 bp, + strand Genetic Position: Chr11, 49.82 cM, cytoband C-E1
• Alliance: Ccl2^tm1Tyos page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151874
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph)
• Mutation: Insertion
• Mutation details: A loxP-neo-loxP cassette was inserted in a PstI site upstream of exon 3, and a second loxP site was inserted in an EcoRI site upstream of exon 1. Correct targeting was confirmed by Southern blotting. Supernatant analysis of activated homozygote macrophages demonstrated secretion of protein product was less than 25% of wild-type controls. Similar ELISA analysis demonstrated a decrease in MCP-3 secretion, which is encoded by the nearby Ccl7 gene. This is considered a hypomorph allele. (J:151874)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ccl2 Mutation: 25 strains or lines available

References

• Original: J:151874 Takahashi M, et al., Monocyte chemoattractant protein-1 (MCP-1), not MCP-3, is the primary chemokine required for monocyte recruitment in mouse peritonitis induced with thioglycollate or zymosan A. J Immunol. 2009 Sep 1;183(5):3463-71
• All: 3 reference(s)